full length cyclin d1 Search Results


luciferase reporter plasmid containing the full-length human cyclin d1 3′-utr  (SwitchGear Genomics)
90
SwitchGear Genomics luciferase reporter plasmid containing the full-length human cyclin d1 3′-utr
miR-206 directly targets CCND1 at a specific 3′-UTR site. ( a ) Schematic of putative miR-206 target site within the human CCND1 3′-UTR as predicted by TargetScan and PicTar algorithms. Mutations introduced into the full-length human CCND1 3′-UTR are highlighted in red. ( b ) Relative <t>luciferase</t> activity was measured in 3T3-L1 cells co-transfected with a luciferase reporter construct containing a fragment of the mouse Ccnd1 3′-UTR encompassing the miR-206 binding site or empty pMIR-Report vector, along with miR-ve or miR-206. Data represent relative Firefly values as determined by the ratio of Firefly to Renilla luciferase activity with control miR-ve set as 1. Values are the mean±s.e.m. of three separate experiments. ( c ) Relative luciferase activity was measured in H1299 cells co-transfected with reporter constructs containing human full-length wild-type (WT)- CCND1 3′-UTR or mutant (MUT)- CCND1 3′-UTR along with miR-ve or miR-206. Firefly luciferase units were normalized against Renilla luciferase units to control for transfection efficiency. Percentage Firefly luciferase values were determined with control miR-ve set at 100%. Data represent the mean+s.e.m. of three independent experiments. A paired t -test was used to determine significance between miR-ve and miR-206 transfected cells (* indicates P <0.05).
Luciferase Reporter Plasmid Containing The Full Length Human Cyclin D1 3′ Utr, supplied by SwitchGear Genomics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+length+cyclin+d1/luciferase+reporter+plasmid+containing+the+full+length+human+cyclin+d1+3++utr/pmc05189965-122-0-13
Average 90 stars, based on 1 article reviews
luciferase reporter plasmid containing the full-length human cyclin d1 3′-utr - by Bioz Stars, 2026-10
90/100 stars
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cdna encoding full-length cyclin d1  (Becton Dickinson)
90
Becton Dickinson cdna encoding full-length cyclin d1
miR-206 directly targets CCND1 at a specific 3′-UTR site. ( a ) Schematic of putative miR-206 target site within the human CCND1 3′-UTR as predicted by TargetScan and PicTar algorithms. Mutations introduced into the full-length human CCND1 3′-UTR are highlighted in red. ( b ) Relative <t>luciferase</t> activity was measured in 3T3-L1 cells co-transfected with a luciferase reporter construct containing a fragment of the mouse Ccnd1 3′-UTR encompassing the miR-206 binding site or empty pMIR-Report vector, along with miR-ve or miR-206. Data represent relative Firefly values as determined by the ratio of Firefly to Renilla luciferase activity with control miR-ve set as 1. Values are the mean±s.e.m. of three separate experiments. ( c ) Relative luciferase activity was measured in H1299 cells co-transfected with reporter constructs containing human full-length wild-type (WT)- CCND1 3′-UTR or mutant (MUT)- CCND1 3′-UTR along with miR-ve or miR-206. Firefly luciferase units were normalized against Renilla luciferase units to control for transfection efficiency. Percentage Firefly luciferase values were determined with control miR-ve set at 100%. Data represent the mean+s.e.m. of three independent experiments. A paired t -test was used to determine significance between miR-ve and miR-206 transfected cells (* indicates P <0.05).
Cdna Encoding Full Length Cyclin D1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+length+cyclin+d1/cdna+encoding+full+length+cyclin+d1/pm27130439-43-26-56
Average 90 stars, based on 1 article reviews
cdna encoding full-length cyclin d1 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


miR-206 directly targets CCND1 at a specific 3′-UTR site. ( a ) Schematic of putative miR-206 target site within the human CCND1 3′-UTR as predicted by TargetScan and PicTar algorithms. Mutations introduced into the full-length human CCND1 3′-UTR are highlighted in red. ( b ) Relative luciferase activity was measured in 3T3-L1 cells co-transfected with a luciferase reporter construct containing a fragment of the mouse Ccnd1 3′-UTR encompassing the miR-206 binding site or empty pMIR-Report vector, along with miR-ve or miR-206. Data represent relative Firefly values as determined by the ratio of Firefly to Renilla luciferase activity with control miR-ve set as 1. Values are the mean±s.e.m. of three separate experiments. ( c ) Relative luciferase activity was measured in H1299 cells co-transfected with reporter constructs containing human full-length wild-type (WT)- CCND1 3′-UTR or mutant (MUT)- CCND1 3′-UTR along with miR-ve or miR-206. Firefly luciferase units were normalized against Renilla luciferase units to control for transfection efficiency. Percentage Firefly luciferase values were determined with control miR-ve set at 100%. Data represent the mean+s.e.m. of three independent experiments. A paired t -test was used to determine significance between miR-ve and miR-206 transfected cells (* indicates P <0.05).

Journal: Oncogenesis

Article Title: Selective repression of the oncogene cyclin D1 by the tumor suppressor miR-206 in cancers

doi: 10.1038/oncsis.2014.26

Figure Lengend Snippet: miR-206 directly targets CCND1 at a specific 3′-UTR site. ( a ) Schematic of putative miR-206 target site within the human CCND1 3′-UTR as predicted by TargetScan and PicTar algorithms. Mutations introduced into the full-length human CCND1 3′-UTR are highlighted in red. ( b ) Relative luciferase activity was measured in 3T3-L1 cells co-transfected with a luciferase reporter construct containing a fragment of the mouse Ccnd1 3′-UTR encompassing the miR-206 binding site or empty pMIR-Report vector, along with miR-ve or miR-206. Data represent relative Firefly values as determined by the ratio of Firefly to Renilla luciferase activity with control miR-ve set as 1. Values are the mean±s.e.m. of three separate experiments. ( c ) Relative luciferase activity was measured in H1299 cells co-transfected with reporter constructs containing human full-length wild-type (WT)- CCND1 3′-UTR or mutant (MUT)- CCND1 3′-UTR along with miR-ve or miR-206. Firefly luciferase units were normalized against Renilla luciferase units to control for transfection efficiency. Percentage Firefly luciferase values were determined with control miR-ve set at 100%. Data represent the mean+s.e.m. of three independent experiments. A paired t -test was used to determine significance between miR-ve and miR-206 transfected cells (* indicates P <0.05).

Article Snippet: Luciferase reporter plasmid containing the full-length human cyclin D1 3′-UTR was obtained from Switchgear Genomics (Carlsbad, CA, USA).

Techniques: Luciferase, Activity Assay, Transfection, Construct, Binding Assay, Plasmid Preparation, Control, Mutagenesis